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タイトル: Genomic Control of Upregulation of GRP78 Expression for Promotion of Neurite Elongation and Attenuation of Cell Death via PKA-Mediated Signaling in PC12 Cells
著者名: YAMAZOE, Ryosuke
NISHIHATA, Yoshiki
NAKAGAWA, Kazuma
AOYAMA, Hiroki
SHIMOKE, Koji
著者の別表記: 山添, 亮輔
西畑, 慶紀
中川, 一馬
青山, 大輝
下家, 浩二
キーワード: neurites
survival
PC12
GRP78
gene expression
genomics
epigenetics
関西大学
Kansai University
平成26年度関西大学若手研究者育成経費
論文発行年月日: 2015年11月23日
出版者: OMICS International
雑誌名: Clinical Pharmacology & Biopharmaceutics
巻: 4
号: 4
開始ページ: 1
終了ページ: 4
抄録: Neurodegeneration occurs due to neuronal cell death and subsequently disrupts neuronal networks. In current medicine, methods for interruption of neuronal cell death and avoidance of disruption of neuronal networks have potential as therapy for neurodegenerative disorders. Development of this therapy requires analysis of the molecular mechanism of neurite extension that leads to network formation. Here, we show that forskolin (fsk), an activator of adenylate cyclase, increases the intracellular cAMP concentration and induces neurite outgrowth in PC12 cells. The effect of fsk on neurite outgrowth was diminished by H89, an inhibitor of protein kinase A (PKA), and by PD98059 and U0126, which are inhibitors of the mitogen-activated protein kinase (MAPK) signaling pathway. With fsk treatment in the presence of tunicamycin, an inducer of cell death, the activity of the glucose-regulated protein 78 (GRP78) promoters was upregulated. Interestingly, this effect was completely abolished by H89 and by PD98059 and U0126. This phenomenon was confirmed using a dominant-negative PKA-expressing PC12 cell line, in which the PKA-mediated signaling pathway was completely eliminated. These lines of evidence suggest that GRP78 promotes neuronal elongation that is regulated by fsk and mediated by PKA.
内容記述: This work was supported by SENRYAKU (2013-2017) and KAKENHI (25340104) grants-in-aid from MEXT (Ministry of Education, Culture, Sports, Science and Technology of Japan), and by a Kansai University Grant-in- Aid for Encouragement of Scientists (2014).
資料種別: Journal Article
著作権等: (C) 2015 Yamazoe R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/10112/11352
DOI: 10.4172/2167-065X.1000150
ISSN: 2167065X
著者版フラグ: publisher
出現コレクション:学内研究費-化学生命工学部

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